The dHax3.SRDX protein efciently repressed the transcription of the RD29A::LUC transgene and endogenous RD29A gene in Arabidopsis. We, therefore, propose that the appropriate CRES-T vector should be chosen depending on situations and purposes. the EAR-repression domain (SRDX) to generate a chime-ric repressor that targets the RD29A promoter. These results clearly demonstrate the potential of ARRl -SRDX as a domi-nant repressor of the primary transcriptional response to cytokinin in planta. We found that the HSP terminator increased transcription efficiency or transcript stability in contrast, these factors were negatively affected by the Gateway linker sequence in our vector system. ARR1-SRDX effectively repressed reporter gene acti-vation by all of the B-type ARRs in the absence and presence of cytokinin (Fig.
#SRDX REPRESSOR ACTIVATOR#
However, the HSP terminator compensated for the negative effect of the Gateway sequence and improved the efficiency of CRES-T in all cases tested and resulted in the highest efficiency achieved to date. Chimeric repressors are short 12-amino acid repressor domains, for example SRDX from Arabidopsis, fused to a transcriptional activator to convert it into a. Our test experiments revealed that the CRES-T vector containing the Gateway linker sequence within the transcribed region showed reduced efficiency of CRES-T when compared with the traditional CRES-T vector. In this study, we developed new CRES-T vectors that are efficient and convenient to use by employing the Gateway system, a new vector backbone and a terminator derived from the heat shock protein 18.2 (HSP) gene. fusion to the SRDX repression domain (SUPERMAN Repression The MYC3 and MYC4 transcription factors are closely related Domain X Hiratsu et al., 2003, 2004). However, the traditional CRES-T vectors are inconvenient for gene cloning and promoter exchange. activates the expression of JAZs and several JA biosynthetic a transcription factor is converted to a chimeric repressor by genes (Chini et al., 2007 Thines et al., 2007 Hou et al., 2010). For CRES-T, a transcription factor is converted to a strong repressor by fusion with an SRDX repression domain, which is then expressed in plants to induce a loss-of-function phenotype. The ectopic expression of AIF-C caused a male-sterile phenotype with indehiscent anthers throughout flower development in Arabidopsis. Chimeric repressor gene-silencing technology (CRES-T) is a powerful tool that has recently been developed for the functional analysis of plant transcription factors and for the genetic manipulation of plant traits.